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KMID : 1149220210290020071
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Korean Journal of Oriental Medical Prescription
2021 Volume.29 No. 2 p.71 ~ p.80
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Cellular-protective effects of Nardotidis seu Sulculii Concha Extract against oxidative stress
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Kim Kwang-Yeon
Lee Seung-Jin
Jee Seon-Young
Bae Su-Jin
Song Yu-Rim
Yun Un-Jung
Bak Seon-Been
Song Jong-Kuk
Son Tae-Jin
Son Jae-Dong
Kim Woo-Hyun
Yang Ju-Hye
Park Sun-Dong
Kim Sang-Chan
Kim Young-Woo
Park Kwang-Il
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Abstract
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Objectives : This study investigated cellular-protective effects of Nardotidis seu Sulculii Concha water extract (NSCE) against oxidative stress induced by arachidonic acid (AA)+iron or tert-butylhydroperoxide (tBHP).
Methods : In vitro, MTT assay was assessed for cell viability, and immunoblotting analysis was performed to detect expression of AMP-activated kinase (AMPK) signaling pathway and autophagy related proteins. In vivo, mice were orally administrated with the aqueous extract of NSCE of 500 mg/kg for 3 days, and then injected with CCl4 0.5 mg/kg body weight to induce acute damage. The level of liver damage was measured by serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) analysis.
Results : Treatment with NSCE inhibited cell death induced by AA+iron and tBHP. NSCE induced the phosphorylation of AMPK, and this compound also induced the phosphorylation of LKB1, an upstream kinase of AMPK, and Acetyl-CoA carboxylase (ACC), a primary downstream target of AMPK. NSCE increased the protein levels of autophagic markers (LC3II and beclin-1) and decreased the phosphorylation of mammalian target of rapamycin (mTOR) and simultaneously increased the phosphorylation of unc-51-like kinase-1 (ULK-1) in time-dependent manner.
Conclusions : NSCE has the ability 1) to protect cells against oxidative stress induced by AA+iron or tBHP. NSCE 2) to activate AMP-activated protein kinase (AMPK), and 3) to regulate autophagy, an important regulator in cell survival.
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KEYWORD
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Nardotidis seu Sulculii Concha, oxidative stress, cell injury, cellular-protective effect
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